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FLUORODENSITOMETRIC ASSAY OF PATULIN IN APPLE PRODUCTS
Author(s) -
SALEM T. F.,
SWANSON B. G.
Publication year - 1976
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1976.tb14428.x
Subject(s) - patulin , chromatography , chemistry , ethyl acetate , thin layer chromatography , silica gel , column chromatography , quantitative analysis (chemistry) , extraction (chemistry) , food science , mycotoxin
ABSTRACT A fluorodensitometric assay was developed for quantitative analysis of patulin in apple products. The procedure involves extraction of patulin from apple products with ethyl acetate, cleanup with silica gel column chromatography and preparative thin‐layer chromatography. A fluorescent derivative, obtained by exposure of patulin on thin‐layer chromatographic plates to concentrated ammonia fumes, permits convenient quantitative fluorodensitometric assay of patulin. The detection limits were 0.1 μg of pure patulin and 1.0 mg of patulin per liter of apple juice. The mean fluorodensitometric recovery of patulin from apple juice was 97.5%.