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ESTIMATION OF CHLOROPHYLL AND CAROTENOID CONTENTS OF WHOLE TOMATO BY LIGHT ABSORBANCE TECHNIQUE
Author(s) -
WATADA ALLEY E.,
NORRIS KARL H.,
WORTHINGTON JOHN T.,
MASSIE DAVIS R.
Publication year - 1976
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1976.tb00611.x
Subject(s) - absorbance , lycopene , carotenoid , chlorophyll , pigment , chemistry , carotene , food science , chlorophyll a , analytical chemistry (journal) , botany , chromatography , biology , biochemistry , organic chemistry
The relationship between optical measurements of whole tomato and pigment content was studied. The difference between the absorbances (A) at 710 and 780 nm [ΔA (710–780 nm)] correlated significantly (r = 0.98) with chlorophyll contents of 0.3–13.4 μg/g fresh weight (gfw). The ΔA (570–780 nm) correlated significantly (r = 0.97) with lycopene contents of 0.2–46.7 μg/gfw. The ΔA (550–580 nm) correlated significantly with the β‐carotene, content (r = 0.95). The reflectance measurements correlated with the chlorophyll and lycopene contents, but the coefficients were lower and the standard errors were larger than those observed with the absorbance measurements.