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SYMPOSIUM: Immobilized Enzymes in Food Systems
Author(s) -
LEE YOON Y.,
TSAO GEORGE T.
Publication year - 1974
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1974.tb17952.x
Subject(s) - immobilized enzyme , diffusion , enzyme , substrate (aquarium) , chemistry , inert , kinetics , macromolecule , chemical engineering , enzyme kinetics , combinatorial chemistry , thermodynamics , biochemistry , organic chemistry , active site , biology , physics , ecology , quantum mechanics , engineering
Intrinsic kinetics of an immobilized enzyme can be different from that of soluble enzyme due to changes of the macromolecule caused by immobilization. Also transport of substrate to enzymic sites on the inert support creates a hindrance to overall reaction. Such diffusional effects on immobilized enzyme reactions were analyzed in terms of effectiveness factor and film factor employing the theories developed earlier in heterogeneous catalysis. The pore diffusion effect in the design of an integral reactor was analytically treated. A brief discussion was given to the diffusional effects in relation to common kinetic studies of immobilized enzymes.