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INACTIVATION AND REGENERATION OF IMMOBILIZED CATALASE
Author(s) -
WANG S. S.,
GALLILI G. E.,
GILBERT S. G.,
LEEDER J. G.
Publication year - 1974
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1974.tb02889.x
Subject(s) - catalase , chemistry , incubation , regeneration (biology) , residence time (fluid dynamics) , chromatography , phosphate buffered saline , enzyme , biochemistry , biology , microbiology and biotechnology , geotechnical engineering , engineering
Catalase was immobilized on collagen membrane. The inactivation of immobilized catalase by 0.01M and 0.1M H 2 O 2 was reported. After the initial stage of inactivation, a stable catalatic activity as measured in a continuous flow of 0.01M H 2 O 2 through a modular reactor was observed for longer than 20 days (2.6 min residence time). The regeneration of catalatic activity from the O.1M H 2 O 2 inactivated catalase occurred after incubating the inactivated modular reactor with 0.01M phosphate buffer, pH 6.8. The amount of activity regenerated is directly proportional to the time of incubation.