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MODE OF ACTION OF ETHYLENE OXIDE ON SPORES OF Clostridium botulinum 62A
Author(s) -
WINARNO F. G.,
STUMBO C. R.
Publication year - 1971
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1971.tb15554.x
Subject(s) - spore , guanine , mode of action , ethylene oxide , alkylation , dna , purine , clostridium botulinum , pyrimidine , chemistry , rna , kinetics , biochemistry , microbiology and biotechnology , biology , nucleotide , toxin , organic chemistry , catalysis , gene , enzyme , polymer , physics , quantum mechanics , copolymer
SUMMARY— Death of spores of Clostridium botulinum when exposed to gaseous ethylene oxide followed first order kinetics. Supplementation of a synthetic medium with the purine and pyrimidine bases of DNA and RNA indicated, as judged by outgrowth from spores which had received sublethal ETO treatments, that the lethal action of ETO on the spores was through alkylation of the guanine and adenine components of DNA. Observed impairment of RNA and protein synthesis was considered an indirect effect resulting from alkylation of DNA components; however, additional evidence bearing on this point is needed to support a more definite conclusion.