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EFFECT OF EDTA ON THE GERMINATION OF AND OUTGROWTH FROM SPORES OF Clostridium botulinum 62‐A
Author(s) -
WINARNO F. G.,
STUMBO C. R.,
HAYES K. M.
Publication year - 1971
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1971.tb03305.x
Subject(s) - spore , germination , clostridium botulinum , chemistry , spore germination , inhibitory postsynaptic potential , food science , microbiology and biotechnology , incubation , toxin , biochemistry , chromatography , botany , biology , neuroscience
SUMMARY –EDTA, in concentrations above 2.5 mM, was found inhibitory to germination of and outgrowth from spores of C. botulinum Type A and to toxin production in a fish homogenate. Inhibitory action was influenced by pH of the medium in the range pH 6.5–8.1, the action increasing with pH. It was influenced by Mg and Ca concentrations in the medium, equimolar concentrations of added CaCl 2 or MgCl 2 , completely erasing the growth inhibitory action. Initial spore concentration also influenced inhibitory efficacy–the higher the spore concentration, the higher the EDTA concentration required for inhibition. There was no evidence that EDTA, in any concentration used, promoted spore germination. Release of Ca, Mg and DPA from incubating spores was suppressed to varying extents by 5.0 and 10 mM EDTA.