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COMPARATIVE GROWTH OF CLOSTRIDIUM PERFRINGENS IN CARBON DIOXIDE AND NITROGEN ATMOSPHERES
Author(s) -
PAREKH KANTILAL G.,
SOLBERG MYRON
Publication year - 1970
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1970.tb12127.x
Subject(s) - carbon dioxide , clostridium perfringens , nitrogen , atmosphere (unit) , chemistry , carbon dioxide in earth's atmosphere , carbon fibers , modified atmosphere , environmental chemistry , food science , materials science , biology , bacteria , organic chemistry , genetics , physics , composite number , shelf life , composite material , thermodynamics
SUMMARY: Relative growth rates of 8 food poisoning strains of Clostridium perfringens in 100% nitrogen atmosphere and in a 100% carbon dioxide atmosphere at 760‐mm pressure were determined. Growth was measured by the pour plate method. Substrate was fluid thioglycollate medium flushed with 100% nitrogen gas or 100% carbon dioxide gas at atmospheric pressure and incubation was at 43° C Headspace gas in the culture flask was analyzed during the growth period. The nitrogen was reduced at the end of 5 hr but the carbon dioxide remained at approximately 100% during the 3.5‐4‐hr exponential growth phase. Generation time of the Clostridium perfringens strains grown in 100% nitrogen atmosphere varied from 12.9–16.9 min and in the 100% carbon dioxide atmosphere from 12.9–17.2 min. When comparing individual strains in carbon dioxide versus nitrogen, no significant difference was noticed. When each of the 8 strains was compared with one another in a nitrogen atmosphere, as well as in a carbon dioxide atmosphere, significant differences were noticed in growth rates.