Premium
Bisulfite Effect on the Chemistry and Activity of Horseradish Peroxidase
Author(s) -
EMBS R. J.,
MARKAKIS P.
Publication year - 1969
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1969.tb12070.x
Subject(s) - bisulfite , horseradish peroxidase , chemistry , peroxidase , cyanide , azide , fluoride , heme , dissociation constant , inorganic chemistry , biochemistry , organic chemistry , enzyme , gene expression , receptor , dna methylation , gene
SUMMARY: Bisulfite retarded the inactivaton of horseradish peroxidase by weak acids. Spectral analysis indicated that the retardation was accomplished by the stabilization of the linkage between the iron containing prosthetic group and the protein. Cyanide, azide and fluoride, which form reversible complexes with peroxidase iron, exerted a similar effect; thus, it is inferred that bisulfite also forms a complex with peroxidase iron. A kinetic method was used to calculate a dissociation constant of 0.2M for the bisulfite peroxidase complex.