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Variations in Recovery of Clostridium perfringens on Commercial Sulfite‐Polymyxin‐Sulfadiazine (SPS) Agar
Author(s) -
HAUSCHILD A. H. W.,
ERDMAN I. E.,
HILSHEIMER R.,
THATCHER F. S.
Publication year - 1967
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1967.tb09714.x
Subject(s) - agar , clostridium perfringens , food science , microbiology and biotechnology , chemistry , phosphate buffered saline , agar plate , chromatography , biology , bacteria , genetics
SUMMARY— Liquid cultures of several strains of Clostridium perfringens type A were diluted with phosphate buffer and enumerated on laboratory‐prepared sulfite‐polymyxin‐sulfa‐diazine (SPS) agar, four lots of commercial SPS agar, and on non‐selective media. Recoveries on laboratory‐prepared SPS agar and on one lot of commercial SPS agar were the same as on non‐selective media. Recoveries on three commercial lots of SPS agar were significantly lower. Replacement of phosphate buffer with 0.1% peptone allowed quantitative recoveries of C. perfringens on three out of four lots of commercial SPS agar, irrespective of whether the cells were grown in liquid cultures or on meat and meat products.