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Immunofluorescent Detection of Staphylococcal Enterotoxin B II. Detection in Foods
Author(s) -
GENIGEQRGIS C.,
SADLER W. W.
Publication year - 1966
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1966.tb01912.x
Subject(s) - enterotoxin , detection limit , fluorescein isothiocyanate , toxin , chemistry , chromatography , microbiology and biotechnology , antibody , staphylococcus aureus , fluorescence , biology , bacteria , escherichia coli , biochemistry , immunology , physics , genetics , quantum mechanics , gene
SUMMARY Specific staphylococcal enterotoxin B anti‐serum conjugated with fluorescein isothiocyanate was used successfully for detecting toxin in food smears or food extracts in about 4–5 hr. The fluorescent antibody technique detected minute amounts of enterotoxin—much less than 1 or 0.05 μg/ml, which are the sensitivity limits of single or double gel diffusion tube tests—‐without involving any extraction procedures.