Premium
Thiobarbituric‐Acid‐Reactive Substances from Autoxidized or Ultraviolet‐Irradiated Unsaturated Fatty Esters and Squalene
Author(s) -
KWON T. W.,
OLCOTT H. S.
Publication year - 1966
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1966.tb01903.x
Subject(s) - chemistry , squalene , thiobarbituric acid , chromatography , fatty acid , sephadex , elution , ultraviolet , irradiation , organic chemistry , lipid peroxidation , enzyme , physics , quantum mechanics , nuclear physics
SUMMARY The water‐soluble 2‐thiobarbituric.acid‐reactive substances (TBRS) from oxidized and UV‐irradiated unsaturated fatty acid esters and squalene were fractionated on Sephadex G‐10 columns, and the fractions were characterized by elution volume, UV‐absorption spectra, and behavior on TLC plates. By these criteria the main component in all eases was unequivocally identified as malonaldehyde (MA). Other TBRS accompanied MA but were clearly distinguishable. They were not identified, how. ever. With squalene, the total TBRS per double bond were only one‐sixth of those of the fatty acid esters, and MA accounted for about half.