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Immunofluorescent Detection of Staphylococcal Enterotoxin B I. Detection in Culture Media
Author(s) -
GENIGEORGIS C.,
SADLER W. W.
Publication year - 1966
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1966.tb00519.x
Subject(s) - enterotoxin , toxin , microbiology and biotechnology , staphylococcus aureus , staining , chemistry , cholera toxin , incubation , conjugate , chromatography , bacteria , biology , biochemistry , escherichia coli , mathematical analysis , genetics , mathematics , gene
SUMMARY Application of the fluorescent antibody technique ( FAT ) to detection of staphylococcal enterotoxin B and of the cells that produce it in culture media was investigated. Enterotoxin B was detected by FAT in culture media both in the presence and in the absence of bacterial cells. Two methods of detection were developed. The first involved staining of fixed smears with fluorescein‐isothiocyanate‐conjugated anti‐enterotoxin B serum. The second technique involved the precipitation of enterotoxin by mixing one drop of fluid containing either living cells and toxin or only toxin with two drops of serially diluted conjugate. After incubation, the formed precipitates were caught on Millipore filter membranes and washed, and impression smears were made on slides. Enterotoxin B was demonstrated with the first technique only when there was a minimum of 15 fig/ml. The second technique detected as little as 1 fig toxin/m1.