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Evaluation of Factors Affecting the Determination of Nitrogen in Soya Products by the Biuret and Orange‐G Dye‐Binding Methods
Author(s) -
POMERANZ Y.
Publication year - 1965
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1965.tb00307.x
Subject(s) - biuret test , kjeldahl method , chemistry , nitrogen , chromatography , orange (colour) , extraction (chemistry) , food science , urea , biochemistry , organic chemistry
SUMMARY Nitrogen content of soya protein was determined by a modification of the biuret method in which protein extraction and color development occur simultaneously in an alkaline copper tartarate solution and by the orange‐G dye‐binding method. Percentage of total nitrogen content extracted into the solution increased as total nitrogen content increased. Amount of extracted nitrogen was reduced in coarsely ground or severely heated samples. In finely pulverized and untoasted samples, correlation of Kjeldahl protein with the biuret method of protein estimation was 0.985, and with the dye binding method was 0.989.