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A Method for Determining 5′‐Nucleotides a
Author(s) -
LENTO HARRY G.,
FORD JAMES A.,
DENTON ARNOLD E.
Publication year - 1964
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1964.tb01757.x
Subject(s) - nucleotide , chemistry , formic acid , formate , phosphate , elution , chromatography , aqueous solution , ribonucleotide , periodate , inosine , organic chemistry , adenosine , biochemistry , catalysis , gene
SUMMARY A method is described for the separation and quantitative determination in a variety of foods of the following 5′‐nucleotides; eytidine‐5′‐phosphate, adenosine‐5′‐phosphate, uridine‐5′‐phosphate, inosine‐5′‐phosphate, and guan‐osine‐5′‐phosphate. This procedure employs a Dowex 1 ion‐exchange resin in the formate form to adsorb and concentrate the nucleotides from an aqueous extract of the food sample. The nucleotides are separated and eluted in the order previously given by means of a gradient elution system, consisting of water—formic acid‐sodium formate. The method gives complete resolution of the 5′‐nucleotides from each other, but not from their corresponding 2′‐ and 3′‐forms. Therefore, following chromatographic separation, the 5′‐nucleotides are determined calorimetrically in the presence of the 2′‐ and 3′‐nncleotides by oxidation with periodate and reaction of the oxidation products with 2, 4‐dinitrophenylhydrazine.