Premium
Chromatographic Fractionation of Sarcoplasmic Proteins of Beef Skeletal Muscle on Ion‐Exchange Cellulose a, b
Author(s) -
FUJIMAKI M.,
DEATHERAGE F. E.
Publication year - 1964
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1964.tb01737.x
Subject(s) - chemistry , fractionation , chromatography , cellulose , sarcoplasm , ion chromatography , elution , ion exchange , denaturation (fissile materials) , skeletal muscle , phosphate , effluent , biochemistry , ion , endoplasmic reticulum , organic chemistry , nuclear chemistry , biology , anatomy , environmental engineering , engineering
SUMMARY Sarcoplasmic proteins from beef skeletal muscle were fractionated by chromatography on ion‐exchange cellulose (cellulose phosphate and diethyl‐aminoethyl cellulose) at pH 5 and 9.3. From effluent diagrams of sarcoplasma from muscle immediately after slaughter, at least 14 fractions were recognized, and some of them were identified with known proteins by assays for enzymic activities. The numbers and levels of eluted peaks in the effluent diagram were found to decrease with storage (aging) of muscle and freeze‐drying of sarcoplasma; these decreases are perhaps due to denaturation. Proteins in solutions appear to be more completely separated by chromatography when different combinations of ion‐exchange cellulose are used.