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Detection of Staphylococcus Enterotoxin I. Flotation Antigen‐Antibody System
Author(s) -
HOPPER SAMUEL H.
Publication year - 1963
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1963.tb00245.x
Subject(s) - enterotoxin , chromatography , chemistry , rhodamine b , toxin , drop (telecommunication) , suspension (topology) , microbiology and biotechnology , biochemistry , escherichia coli , biology , telecommunications , mathematics , photocatalysis , homotopy , computer science , pure mathematics , gene , catalysis
SUMMARY Staphylococcus enterotoxin B has been concentrated and removed from aqueous solution by froth flotation. An anionic wetting agent and an enterotoxin B antiserum labeled with Rhodamine are added to the toxin solution. Compressed air is blown through the mixture and a foam is produced. The dye‐toxin complex, located in the top layer of the foam, separates from the remainder to give a distinctly red‐colored fraction. With a slight increase in air pressure, the red fraction is removed. The toxin is identified by taking' one drop of the foam fraction, one drop of buffer at pH 4.5, and one drop of latex polystyrene suspension. Within 1 min a heavy agglutination occurs.
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