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Modification of the Pad‐plate Method of Determining Chlortetracycline in Egg White
Author(s) -
BOYD JOSEPHINE W.,
WEISER H. H.,
HAQUE RIAZ UL,
WINTER A. R.
Publication year - 1961
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1961.tb00779.x
Subject(s) - chlortetracycline , diluent , phosphate buffered saline , chromatography , egg white , chemistry , plate count , incubation , antibiotics , food science , biology , biochemistry , bacteria , nuclear chemistry , genetics
SUMMARY The sensitivity of the pad‐plate method for detecting chlortetracycline in egg white is increased by using pH 4.5 citrate buffer rather than pH 4.5 phosphate buffer as a diluent. It is also increased by allowing about four hours for diffusion of antibiotic prior to incubation of the assay plates. Under these conditions of assay, protein binding of the antibiotic was not an important factor.