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Bilobar colorectal liver metastases: a new model for preclinical studies
Author(s) -
Maggiori Léon,
Bretagnol Frédéric,
Wagner Mathilde,
Hatwell Caroline,
Panis Yves
Publication year - 2012
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/j.1365-2613.2012.00841.x
Subject(s) - capsule , medicine , liver lobe , colorectal cancer , metastasis , pathology , lung , cancer , biology , botany
Summary This study aimed to develop a new model of colorectal liver metastases ( LM ) in the rat. Both single macroscopic and multiple bilobar microscopic LM were investigated, as this closely resembled the human situation, before right hepatectomy was performed for ‘single’ right LM . The single macroscopic LM was elicited by direct injection of DHD /K12 colorectal cancer cells under the capsule of the median liver lobe in immunocompetent BDIX rats. The bilobar micrometastases were elicited by intraportal injection of DHD /K12 cells. A preliminary protocol was conducted to assess the dose of cells required to inject in to the portal vein, using 10 6 , 2 × 10 6 and 3 × 10 6 DHD /K12 cells ( n = 15 rats). The resultant protocol for the experimental model used intraportal injection of 10 6 DHD /K12 cells and direct injections of 0.5 × 10 6 , 10 6 and 1.5 × 10 6 DHD /K12 cells ( n = 15 rats). For both protocols, BDIX rats were sacrificed at day 30 after injection. The preliminary protocol showed that intraportal injection of 10 6 DHD /K12 cells was associated with bilobar micrometastases of 0.8 mm mean diameter at day 30. The main protocol assessed that direct injection of 0.5 × 10 6 under the liver median lobe capsule and intraportal injection of 10 6 DHD /K12 cells were associated at day 30 with a single macroscopic metastasis confined to a liver lobe and bilobar micrometastases, without peritoneal carcinomatosis or lung metastasis. Thus we have developed a new experimental model of bilobar colorectal LM including both macro‐ and microscopic colorectal LMs, which mimics the human situation and which will be useful in preclinical studies.