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Analysis of mi R ‐376 RNA cluster members in the mouse inner ear
Author(s) -
Yan Denise,
Xing Yazhi,
Ouyang Xiaomei,
Zhu Juhong,
Chen Zhengyi,
Lang Hainan,
Liu Xue Z.
Publication year - 2012
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/j.1365-2613.2012.00840.x
Subject(s) - inner ear , biology , rna , in situ hybridization , gene , gene expression , vestibular system , microbiology and biotechnology , genetics , anatomy , neuroscience
Summary Mutations in phosphoribosyl pyrophosphate synthetase 1 ( PRPS 1 ) are associated with a spectrum of non‐syndromic to syndromic hearing loss. PRPS 1 transcript levels have been shown to be regulated by the micro RNA ‐376 genes. The long primary RNA transcript of the mi R ‐376 RNA cluster members undergo extensive and simultaneous A → I editing at one or both of two specific sites (+4 and +44) in particular human and mouse tissues. The PRPS 1 gene, which contains target sites for the edited version of mi R ‐376a‐5p within its 3′ UTR , has been shown to be repressed in a tissue‐specific manner. To investigate whether the transcription of Prps1 is regulated by mi R ‐376 cluster members in the mouse inner ear, we first quantified the expression of the mature mi R ‐376 RNA s by quantitative real‐time‐ PCR . The spatio‐temporal patterns of mi R ‐376 expression were assessed by in situ hybridization. Finally, we examined whether A →I editing of pri‐mi R ‐376 RNA s occurs in mouse inner ear by direct sequencing. Our data showed that the mi R ‐376a‐3p, b‐3p, c‐3p are present in mouse embryonic inner ears and intensive expression of mi R ‐376a‐3p/b‐3p was detected in the sensory epithelia and ganglia of both auditory and vestibular portions of the inner ear. In adult inner ear, the expression of mi R ‐376a‐3p/b‐3p is restricted within ganglion neurons of auditory and vestibular systems as well as the cells in the stria vascularis. Only unedited pri‐mi R ‐376 RNA s were detected in the cochlea suggesting that the activity of PRPS 1 in the inner ear may not be regulated through the editing of mi R ‐376 cluster.