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Hepatoprotective effect of B athysa cuspidata in a murine model of severe toxic liver injury
Author(s) -
Gonçalves Reggiani V.,
Novaes Rômulo D.,
Leite João P. V.,
Vilela Emerson F.,
Cupertino Marli C.,
Nunes Líria G.,
Matta Sérgio L. P.
Publication year - 2012
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/j.1365-2613.2012.00835.x
Subject(s) - carbon tetrachloride , liver injury , superoxide dismutase , chemistry , alanine transaminase , aspartate transaminase , bark (sound) , ccl4 , transaminase , dimethyl sulfoxide , pharmacology , traditional medicine , oxidative stress , biochemistry , medicine , enzyme , alkaline phosphatase , biology , ecology , organic chemistry
Summary The objective of this study was to investigate the hepatoprotective effect of a bark extract of B athysa cuspidata extract ( BCE ) in a murine model of severe liver injury induced by carbon tetrachloride ( CC l 4 ). Forty‐two Wistar rats were randomized into six groups of seven animals each: G roup 1( G 1): CC l 4 ; G roup 2 ( G 2): dimethyl sulfoxide ( DMSO ) + CC l 4 ; G roup 3 ( G 3): BCE 400 mg/kg alone; G roup 4 ( G 4): BCE 200 mg/kg + CC l 4 ; G roup 5 ( G 5): BCE 400 mg/kg + CC l 4 ; G roup 6 ( G 6): DMSO alone. The extract was administered by gavage for 18 days beginning 6 days prior to the first application of CC l 4 . After completing CC l 4 administration, the animals were euthanized. The animals in G 1, G 2, G 4 and G 5 experienced significant body weight loss and had an increased liver somatic index compared with G 3 and G 6 ( P < 0.05). A significant reduction in serum aspartate and alanine transaminase and gamma‐glutamyl transferase ( P < 0.05) and a significant increase in the activity of the anti‐oxidant enzyme superoxide dismutase were found in G 5 ( P < 0.05). Lower proportions of cellular necrosis and lipid droplets were found in the livers of animals in G 4 and G 5 compared with G 1 and G 2 ( P < 0.05). These results confirm the marked hepatoprotective activity of the bark extract of B athysa cuspidata in severe injuries induced by CC l 4 in rats and suggest that this effect may be associated with the inhibition of oxidative damage.