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Expression of the reversion‐inducing cysteine‐rich protein with Kazal motifs and matrix metalloproteinase‐14 in neuroblastoma and the role in tumour metastasis
Author(s) -
Dong Qian,
Yu Dan,
Yang ChuanMin,
Jiang Buxian,
Zhang Hong
Publication year - 2010
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/j.1365-2613.2010.00724.x
Subject(s) - neuroblastoma , metastasis , immunohistochemistry , cancer research , matrix metalloproteinase , ganglioneuroma , reversion , biology , n myc , metastasis suppressor , microbiology and biotechnology , pathology , gene , medicine , cancer , immunology , cell culture , phenotype , genetics
Summary Neuroblastoma is the most common malignant tumour in infancy; the reversion‐inducing cysteine‐rich protein with Kazal motifs gene ( RECK ) is a tumour suppressor gene. Previous studies show that RECK inhibits tumour invasion and metastasis through negative regulation of the matrix metalloproteinase (MMP)‐2, MMP‐9 and MMP‐14. Therefore, we wanted to detect the expression of RECK and MMP‐14 in neuroblastomas to assess the correlation between the expression levels of these proteins, and to investigate the roles in the metastasis and development of the tumour. PV‐6000 immunohistochemistry method was used to detect the expression levels of RECK and MMP‐14 in 36 samples of neuroblastoma tissue. Samples from paraffin wax‐embedded specimens and the complete clinicopathological data of 36 neuroblastoma and 10 ganglioneuroma patients were collected. The rate of expression of the RECK protein in the neuroblastoma was low (16.7%). Furthermore, it reduced with the increase in the invasive depth and distant metastasis ( P = 0.015; P < 0.05). The rate of expression of the MMP‐14 protein in the neuroblastoma was high (58.3%) and increased with the increase in the extent of invasive depth and distant metastasis ( P = 0.002; P < 0.05). The expression of the RECK protein correlated negatively with that of MMP‐14 ( r = −0.418; P < 0.05). Low levels of the RECK protein are expressed in the neuroblastoma, while the MMP‐14 protein is expressed at high levels. The RECK and MMP‐14 proteins may serve as markers in the estimation of the extent of metastasis and dissemination of the neuroblastoma.