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Identification of monoclinic calcium pyrophosphate dihydrate and hydroxyapatite in human sclera using Raman microspectroscopy
Author(s) -
Chen KoHua,
Li MeiJane,
Cheng WenTing,
BalicZunic Tonci,
Lin ShanYang
Publication year - 2009
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/j.1365-2613.2008.00614.x
Subject(s) - raman spectroscopy , monoclinic crystal system , raman microspectroscopy , sclera , calcification , triclinic crystal system , chemistry , crystallography , materials science , pathology , medicine , crystal structure , ophthalmology , optics , physics
Summary Raman microspectroscopy was first used to determine the composition of a calcified plaque located at the pterygium‐excision site of a 51‐year‐old female patient’s left nasal sclera after surgery. It was unexpectedly found that the Raman spectrum of the calcified sample at 1149, 1108, 1049, 756, 517, 376 and 352/cm was similar to the Raman spectrum of monoclinic form of calcium pyrophosphate dihydrate (CPPD) crystal, but differed from the Raman spectrum of triclinic form of CPPD. An additional peak at 958/cm was also observed in the Raman spectrum of the calcified plaque, which was identical to the characteristic peak at 958/cm of hydroxyapatite (HA). This is the first study to report the spectral biodiagnosis of both monoclinic CPPD and HA co‐deposited in the calcified plaque of a patient with sclera dystrophic calcification using Raman microspectroscopy.