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Intratubular transplantation as a strategy for establishing animal models of testicular germ cell tumours
Author(s) -
Li Yunmin,
Kido Tatsuo,
Luo Jinping,
Fukuda Michiko,
Dobrinski Ina,
Lau YunFai Chris
Publication year - 2008
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/j.1365-2613.2008.00611.x
Subject(s) - transplantation , biology , transgene , germ cell , testicular cancer , cancer research , stem cell , animal model , pathogenesis , cell , cancer , gene , immunology , pathology , medicine , genetics , endocrinology
Summary Testicular germ cell tumours (TGCTs) are prevalent cancers among young men. Currently, there is no reliable animal model for TGCTs. To establish such animal models, we have explored the possibility of intratubular testicular transplantation as means to deliver tumour cells into the seminiferous tubules of host animals. Our results demonstrated that transplanted cells could effectively populate the testis of a recipient mouse and develop into TGCTs. In addition, the donor cells could be transfected with a specific transgene before transplantation, thereby providing an approach to evaluate the specific effects of gene functions in the oncogenic processes. Hence, depending on selection of specific donor cells or mixtures of donor cells, transplantation models of TGCTs could be significant for studies on the pathogenesis, diagnosis and therapies of such a prevalent and important cancer in men.