Open AccessInhibition of Leydig Cell Steroidogenesis: Effect of Actinomycin D Before and After Preincubation of Leydig Cells In Vitro
Author(s) -
Cooke B. A.,
Janszen F. H. A.,
Driel M. J. A.
Publication year - 1978
Publication title -
international journal of andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 43
eISSN - 1365-2605
pISSN - 0105-6263
DOI - 10.1111/j.1365-2605.1978.tb00019.x
Subject(s) - stimulation , incubation , leydig cell , in vitro , testosterone (patch) , medicine , endocrinology , messenger rna , dactinomycin , cell culture , biology , chemistry , protein biosynthesis , microbiology and biotechnology , biochemistry , hormone , luteinizing hormone , cycloheximide , gene , genetics
The effect of preincubating purified Leydig cells in Eagle's medium and the subsequent effect of the mRNA synthesis inhibitor actinomycin D on LH‐stimulated testosterone synthesis has been investigated. The inhibitory effect obtained was found to decrease with the period of preincubation; with 0, 1, 2 and 3 h preincubation before the addition of LH (100 ng/ml) 36.5 ± 3.9, 31.2 ± 2.5, 17.8 ± 3.8 and 13.6 ± 2.9% inhibition occurred respectively when actinomycin D (6.4 μM) was added and the cells were incubated for 2 h (means ± SEM, n = 5). During the first hour of incubation with LH and actinomycin D no inhibition occurred in cells that had been preincubated for 3 h. These results suggest that during preincubation and independently of LH, synthesis of intermediates (possibly mRNA(s)) required for stimulation of steroidogenesis may take place and that subsequent stimulation of steroidogenesis by LH occurs without further de novo mRNA synthesis.