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Effect of three different storage media on survival of periodontal ligament cells using collagenase–dispase assay
Author(s) -
Mahal N. K.,
Singh N.,
Thomas A. M.,
Kakkar N.
Publication year - 2013
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2012.02129.x
Subject(s) - dispase , trypan blue , hemocytometer , collagenase , periodontal fiber , balanced salt solution , viability assay , propolis , andrology , significant difference , dentistry , biology , chemistry , cell , food science , biochemistry , medicine , enzyme , organic chemistry
Aim To evaluate and compare the efficacy of propolis, egg albumen and H ank's balanced salt solution ( HBSS ) in maintaining the viability of human periodontal ligament ( PDL ) cells using a collagenase–dispase assay. Methodology Fifty‐five freshly extracted human teeth were divided into three experimental ( HBSS , egg albumen and propolis) and two control groups. Fifteen teeth per experimental group were stored dry for 30 min and then immersed for 45 min in one of the three experimental media. The positive and negative controls corresponded to 0‐min and 1‐h dry time, respectively, with five teeth per control group. The teeth were then treated with collagenase II and dispase II for 30 min and labelled with 0.4% trypan blue for determination of viability. The number of viable cells was counted with a haemocytometer and analysed statistically by anova and post hoc Tukey HSD test. Results Statistical analysis demonstrated there was no significant difference between HBSS , egg albumen and propolis in maintaining cell viability. Conclusion Egg albumen and propolis may be able to maintain PDL cell viability as well as HBSS .