Premium
Polymorphisms of pro‐inflammatory cytokine genes and the risk for acute suppurative or chronic nonsuppurative apical periodontitis in a C olombian population
Author(s) -
Amaya M. P.,
Criado L.,
Blanco B.,
Gómez M.,
Torres O.,
Flórez L.,
González C. I.,
Flórez O.
Publication year - 2013
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2012.02097.x
Subject(s) - interleukin 8 , genotype , medicine , genotyping , interleukin , chronic periodontitis , immunology , allele , single nucleotide polymorphism , gastroenterology , periodontitis , cytokine , biology , gene , genetics
Aim To determine the association of functional single nucleotide polymorphisms in genes of the pro‐inflammatory cytokines tumour necrosis factor‐α, interleukin‐1β, interleukin‐8 and interleukin‐12 B with the development of two clinical forms of apical periodontitis ( AP ): acute suppurative and chronic nonsuppurative. Methodology The study included 120 patients from B ucaramanga City, C olombia, 63 diagnosed with acute suppurative AP ( ASAP ) and 57 diagnosed with chronic nonsuppurative AP ( CNAP ). Genotyping for IL1B +3954 (rs1143634), IL8 / CXCL8 −251 (rs4073), IL12B +1188 (rs3212227) and TNFA −308 (rs1800629) was performed by the PCR –restriction fragment length polymorphisms method. The statistical analysis was performed using STATA 10.0 and PLINK V 1.07 software. Results Significant differences in the distribution of IL8 / CXCL8 −251 A allele ( P adjusted = 0.041; OR adjusted = 0.41, CI adjusted = 0.31–0.97) and IL8 / CXCL −251 TT genotype ( P adjusted = 0.04; OR adjusted = 2.24, CI adjusted = 1.04–4.84) were observed comparing patients diagnosed with ASAP and CNAP . No association was observed in genotype and allele distribution for other genetic polymorphisms analysed. Conclusion This study provides molecular epidemiological evidence that suggests in the present cohort that IL8 / CXCL8 −251 T allele, which is associated with higher production of IL 8/ CXCL 8, is also associated with a higher risk of developing acute suppurative form of AP , whereas IL8 / CXCL8 −251 A allele, which is associated with lower production of IL 8/ CXCL 8, is associated with chronic nonsuppurative form of AP . This suggests a pivotal role for IL ‐8/ CXCL 8 in periapical disease because of its ability to induce chemotaxis and modulating the directed migration of neutrophils to the site of inflammation in response to microbial infection of pulp.