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The upregulation of tumour necrosis factor‐α and surface antigens expression on macrophages by bisphenol A‐glycidyl‐methacrylate
Author(s) -
Kuan Y.H.,
Li Y.C.,
Huang F.M.,
Chang Y.C.
Publication year - 2012
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2012.02017.x
Subject(s) - cytotoxicity , tumor necrosis factor alpha , antigen , cd80 , microbiology and biotechnology , chemistry , necrosis , immune system , biology , immunology , in vitro , cytotoxic t cell , medicine , biochemistry , cd40 , pathology
Kuan Y‐H, Li Y‐C, Huang F‐M, Chang Y‐C. The upregulation of tumour necrosis factor‐α and surface antigens expression on macrophages by bisphenol A‐glycidyl‐methacrylate. International Endodontic Journal , 45 , 619–626, 2012. Abstract Aim To evaluate the expression of tumour necrosis factor‐α and surface antigens by bisphenol A‐glycidyl‐methacrylate (BisGMA) on murine macrophage cell line RAW264.7. Methodology Cytotoxicity was measured by tetrazolium bromide reduction assay. Tumour necrosis factor (TNF)‐α was analysed by enzyme‐linked immunosorbent assay. Cell surface antigens were investigated by flowcytometry. Statistical analyses were performed using anova followed by the Bonferroni’s t ‐test for multigroup comparisons. Results BisGMA exhibited cytotoxicity to RAW264.7 in a dose‐dependent manner ( P < 0.05) during 2‐h incubation period. BisGMA was found to increase TNF‐α secretion in a dose‐dependent manner ( P < 0.05). In addition, CD11, CD14, CD45, CD54, CD40, CD80, and MHC II were significantly stimulated by BisGMA in a dose‐dependent manner ( P < 0.05). However, MHC I expression was not affected by BisGMA ( P > 0.05). Conclusions Taken together, the ability of macrophages to induce an appropriate immune response when exposed to BisGMA has the potential to upregulate TNF‐α production and expression of surface antigens.