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Effectiveness of three instrumentation systems to remove Enterococcus faecalis from root canals
Author(s) -
Matos Neto M.,
Santos S. S. F.,
Leão M. V. P.,
Habitante S. M.,
Rodrigues J. R. D. D.,
Jorge A. O. C.
Publication year - 2012
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2011.01994.x
Subject(s) - enterococcus faecalis , root canal , debridement (dental) , dentistry , significant difference , bioburden , sterilization (economics) , mathematics , biology , medicine , surgery , statistics , bacteria , genetics , staphylococcus aureus , monetary economics , economics , foreign exchange market , foreign exchange
Matos Neto M, Santos SSF, Leão MVP, Habitante SM, Rodrigues JRDD, Jorge AOC. Effectiveness of three instrumentation systems to remove Enterococcus faecalis from root canals. International Endodontic Journal , 45 , 435–438, 2012. Abstract Aim To assess the effectiveness of three systems of mechanical preparation to reduce Enterococcus faecalis within root canals. Methodology Twenty‐four human single‐rooted canine teeth were standardized to a length of 17 mm and the canal contents removed using a size 20 K‐file, as the last apical file. After irrigation and sterilization, the canals were contaminated with E. faecalis and incubated for 21 days at 37 °C with 5% CO 2 . Then, the teeth were divided into three groups for mechanical preparation with: ProTaper rotary system, ProTaper manual system and manual K‐files. Samples of the root canal contents, before and after the debridement, were collected with sterile paper points for 1 min. Then, the samples were diluted and plated in Brain Heart Infusion (BHI) agar. The colony‐forming units were counted and the percentage reduction calculated. The reduction and log CFU mL −1 were compared between groups using Wilcoxon nonparametric test and two‐way analysis of variance, respectively. Results There was a significant reduction in the number of CFU/mL ( P = 0.000) before and after debridement for all the systems used. However, there was no significant difference between the systems. Conclusion All the three instrumentation systems reduced E. faecalis counts to a similar degree.