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Effectiveness of calcium hydroxide‐based intracanal medicaments against Enterococcus faecalis
Author(s) -
Lima R. K. P.,
GuerreiroTanomaru J. M.,
FariaJúnior N. B.,
TanomaruFilho M.
Publication year - 2012
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2011.01976.x
Subject(s) - enterococcus faecalis , calcium hydroxide , chlorhexidine , root canal , dentistry , medicine , significant difference , chemistry , biology , staphylococcus aureus , bacteria , genetics
Lima RKP, Guerreiro‐Tanomaru JM, Faria‐Júnior NB, Tanomaru‐Filho M. Effectiveness of calcium hydroxide‐based intracanal medicaments against Enterococcus faecalis. International Endodontic Journal , 45 , 311–316, 2012. Abstract Aim To evaluate the antimicrobial efficacy of calcium hydroxide‐based intracanal medicaments against Enterococcus faecalis . Methodology One hundred and six single‐rooted human teeth were inoculated with E. faecalis ( ATCC 29212 ) and incubated in an microaerophilic environment for 21 days. Teeth were divided according to the medicaments and period: G1 – Calen (7 days); G2 – Calen (14 days); G3 – Calen/camphorated paramonochlorophenol (CMCP) (7 days); G4 – Calen/CMCP (14 days); G5 – Calen/0.4% chlorhexidine (CHX) (7 days); G6 – Calen/0.4% CHX (14 days); G7 – Calen/1% CHX (7 days); G8 – Calen/1% CHX (14 days); G9 – chemo‐mechanical preparation with no medication and G10 – no chemo‐mechanical preparation and no medication. Microbial samples were harvested immediately after removal of the intracanal dressing and 7 days later. Data were subjected to anova ( P < 0.05). Results All medicaments were associated with a reduction in E. faecalis after removal of the dressing (post‐medication harvest). However, all specimens were associated with an increase in CFU mL −1 values after 7 days (final harvest). Calen/CMCP and Calen/CHX had the lowest CFU mL −1 values ( P < 0.05). Conclusion In this laboratory study using extracted teeth all calcium hydroxide‐based medicaments were able to significantly reduce the CFU mL −1 values of E. faecalis in the root canal system.