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Periodontal ligament fibroblast response to root perforations restored with different materials – a laboratory study
Author(s) -
Hakki S. S.,
Bozkurt S. B.,
Ozcopur B.,
Purali N.,
Belli S.
Publication year - 2012
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2011.01968.x
Subject(s) - periodontal fiber , mineral trioxide aggregate , fibroblast , dentistry , mtt assay , materials science , chemistry , molar , microbiology and biotechnology , cell , in vitro , biology , medicine , biochemistry
Hakki SS, Bozkurt SB, Ozcopur B, Purali N, Belli S. Periodontal ligament fibroblast response to root perforations restored with different materials – a laboratory study. International Endodontic Journal , 45 , 240–248, 2012. Abstract Aim To compare the effect of several materials on the attachment of periodontal ligament (PDL) fibroblasts to experimentally perforated root surfaces. Methodology Root specimens (size 5 × 5 mm) were obtained from extracted human molar teeth and perforations with a 1 mm diameter were created. One group was kept as a control and the rest were repaired with the following materials: Amalgam, Dyract, IRM, Super Bond C&B and Mineral trioxide aggregate (MTA). PDL fibroblasts were placed at a density of 8 × 10 4 cells on the root specimens, incubated on tissue culture inserts (48 h) and then transferred to 48 well‐plates. MTT assays were performed at 48 and 96 h for PDL fibroblast survival. Cell attachment was observed using confocal microscopy on days 2 and 5. Total RNAs from the root specimens were isolated on day 5 and type I collagen (COL I) and Runt‐related transcription factor 2 (Runx2) mRNA expressions were checked using Quantitative‐Polymerase Chain Reaction (QPCR). For the MTT assay and QPCR, one‐way analysis of variance ( anova ) and Tukey HSD multiple comparison tests were used to compare the groups. Results Mineral trioxide aggregate resulted in a significantly higher cell density ( P < 0.001). Dyract, IRM and Super Bond C&B groups had a lower cell density when compared with the control and MTA groups at 48 h ( P < 0.001). Confocal microscopy revealed that, among the experimental groups, the MTA group had the largest viable cell population over the restoration site when compared with the other materials; however, reduced cell attachment was noted in all groups when compared with the control. Increased Runx2 mRNA expressions were noted in MTA ( P < 0.001) and IRM ( P < 0.01) groups when compared with control and other tested materials. COL I transcripts were increased in IRM ( P < 0.01), D, C&B and MTA ( P < 0.001) when compared with the control. Conclusion Mineral trioxide aggregate provided a more favorable environment for PDL cell adhesion and growth.