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Overexpression of interleukin‐6 and ‐8, cell growth inhibition and morphological changes in 2‐hydroxyethyl methacrylate‐treated human dental pulp mesenchymal stem cells
Author(s) -
Trubiani O.,
Cataldi A.,
De Angelis F.,
D’Arcangelo C.,
Caputi S.
Publication year - 2012
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2011.01942.x
Subject(s) - mesenchymal stem cell , dental pulp stem cells , pulp (tooth) , ex vivo , stem cell , cell growth , interleukin , cell , andrology , chemistry , immunology , microbiology and biotechnology , cytokine , biology , in vitro , dentistry , medicine , biochemistry
Trubiani O, Cataldi A, De Angelis F, D’Arcangelo C, Caputi S. Overexpression of interleukin‐6 and ‐8, cell growth inhibition and morphological changes in 2‐hydroxyethyl methacrylate‐treated human dental pulp mesenchymal stem cells. International Endodontic Journal , 45 , 19–25, 2012. Abstract Aim To evaluate morphological features, cell growth and interleukin‐6 (IL‐6) and interleukin‐8 (IL‐8) secretion in expanded ex vivo human dental pulp mesenchymal stem cells (DP‐MSCs) after exposure to 2‐hydroxyethyl methacrylate (HEMA). Methodology Dental pulp mesenchymal stem cells were derived from the dental pulps of 10 young donors. After in vitro isolation, DP‐MSCs were treated with 3 and 5 mmol L −1 HEMA, and after 24, 48 and 72 h of incubation, their morphological features, cell growth, IL‐6 and IL‐8 secretion were analysed. Differences in the cell growth and in the interleukin secretion were analysed for statistical significance with two‐way anova tests and the Holm–Sidak method for multiple comparisons. Results Dental pulp mesenchymal stem cells revealed a decrease in cell growth with both treatments ( P < 0.05), more evident at 5mmol L −1 . Microscopic analysis displayed extensive cytotoxic effects in treated cells, which lost their fibroblastoid features and became retracted, even roundish, with a large number of granules. An up‐regulation of IL‐6 and IL‐8 in treated cells cytokines was evident ( P < 0.05). Conclusions 2‐Hydroxyethyl methacrylate exhibited cytotoxicity, inhibited cell growth and induced morphological changes in cultured DP‐MSCs. Moreover, in treated samples, an up‐regulation of soluble mediators of inflammation such as IL‐6 and IL‐8 cytokines was found. The direct application of HEMA potentially induces an inflammation process that could be the starting point for toxic response and cell damage in DP‐MSCs.