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Initial fibroblast attachment to Erbium:YAG laser‐irradiated dentine
Author(s) -
Bolortuya G.,
Ebihara A.,
Ichinose S.,
Watanabe S.,
Anjo T.,
Kokuzawa C.,
Saegusa H.,
Kawashima N.,
Suda H.
Publication year - 2011
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2011.01934.x
Subject(s) - irradiation , fibroblast , laser , er:yag laser , dentin , materials science , dentistry , scanning electron microscope , sterilization (economics) , nuclear chemistry , molar , biomedical engineering , chemistry , medicine , composite material , biochemistry , physics , nuclear physics , monetary economics , economics , optics , in vitro , foreign exchange market , foreign exchange
Bolortuya G, Ebihara A, Ichinose S, Watanabe S, Anjo T, Kokuzawa C, Saegusa H, Kawashima N, Suda H. Initial fibroblast attachment to Erbium:YAG laser‐irradiated dentine. International Endodontic Journal , 44 , 1134–1144, 2011. Abstract Aims To evaluate the effects of Erbium (Er):YAG laser irradiation on the morphology of resected dentine surfaces, and to investigate fibroblast attachment to laser‐irradiated dentine surfaces. Methodology Dentine blocks obtained from single‐rooted human teeth were divided into the following groups after sterilization in an autoclave: (i) Laser group treated with Er:YAG laser irradiation (30 mJ per pulse, 10 pps, 60 s); (ii) L‐MTAD group treated with laser irradiation as in (i) plus a mixture of doxycycline, tetracycline isomer and citric acid; (iii) RC‐Prep group treated with EDTA gel or cream (RC‐Prep) and (iv) Control group left untreated. After each treatment, the dentine blocks were incubated with NIH/3T3 fibroblasts cultured to subconfluency in Dulbecco’s modified Eagle’s medium supplemented with 10% foetal bovine serum and antibiotics. The number of attached cells amongst the groups was analysed statistically at the 5% significance level. The dentine surface morphologies and cell attachments were evaluated by counting assays, histological observations and scanning electron microscopy (SEM). Results The number of attached cells was significantly higher ( P < 0.05) in the Laser group than in the RC‐Prep and Control groups at 16 h. Dendritic cell extension of the fibroblasts was only observed in the Laser group at 8 h by SEM. In the histological analyses, significantly more attached cells were found on the dentine surfaces treated with laser irradiation. Conclusions Er:YAG laser irradiation induced morphological alterations in dentine surfaces, which may improve the attachment of fibroblasts to dentine.