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Bacterial killing by several root filling materials and methods in an ex vivo infected root canal model
Author(s) -
Özcan E.,
Eldeniz A. U.,
Arı H.
Publication year - 2011
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2011.01928.x
Subject(s) - root canal , enterococcus faecalis , materials science , negative control , positive control , dentistry , polydimethylsiloxane , composite material , bacteria , biology , medicine , staphylococcus aureus , genetics , traditional medicine
Özcan E, Eldeniz AU, Arı H. Bacterial killing by several root filling materials and methods in an ex vivo infected root canal model. International Endodontic Journal , 44 , 1102–1109, 2011. Abstract Aim To evaluate the ability of two root canal sealers (Epoxy resin‐based AH Plus ® or polydimethylsiloxane‐based GuttaFlow ® ) and five root filling techniques (continuous wave of condensation, Thermafil ® , lateral condensation, matched taper single gutta‐percha point, laterally condensed‐matched taper gutta‐percha point) to kill bacteria in experimentally infected dentinal tubules. Methodology An infected dentine block model was used. One hundred and twenty extracted, single‐rooted human teeth were randomly divided into 10 test ( n = 10) and 2 control ( n = 10) groups. The roots, except negative controls, were infected with Enterococcus faecalis for 21 days. The root canals were then filled using the test materials and methods. Positive controls were not filled. Sterile roots were used as negative controls. Dentine powder was obtained from all root canals using gates glidden drills using a standard method. The dentine powder was diluted and inoculated into bacterial growth media. Total colony‐forming units (CFU) were calculated for each sample. Statistical analysis was performed using the Kruskal–Wallis and Mann–Whitney U test. Results The epoxy resin‐based sealer was effective in killing E. faecalis except when using Thermafil ( P < 0.05), but the polydimethylsiloxane‐based sealer was not effective in killing this microorganism except in the continuous wave group ( P < 0.05). Conclusions In the test model, AH Plus killed bacteria in infected dentine more effectively than GuttaFlow. The filling method was less important than the sealer material.