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Comparative analysis of two colorimetric assays in dental pulp cell density
Author(s) -
Nedel F.,
Soki F. N.,
Conde M. C. M.,
Zeitlin B. D.,
Tarquinio S. B. C.,
Nör J. E.,
Seixas F. K.,
Demarco F. F.
Publication year - 2011
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2010.01796.x
Subject(s) - sulforhodamine b , correlation coefficient , pulp (tooth) , pearson product moment correlation coefficient , dental pulp stem cells , coefficient of variation , colorimetry , dentistry , chemistry , chromatography , medicine , mathematics , in vitro , biochemistry , statistics , cytotoxicity
Nedel F, Soki FN, Conde MCM, Zeitlin BD, Tarquinio SBC, Nör JE, Seixas FK, Demarco FF. Comparative analysis of two colorimetric assays in dental pulp cell density. International Endodontic Journal , 44 , 59–64, 2011. Abstract Aim To compare and contrast two colorimetric assays used for the measurement of proliferation using two dental pulp cell types: dental pulp stem cells (DPSC) and human dental pulp fibroblasts (HDPF). Methodology Dental pulp stem cells or HDPF were seeded at 0.25 × 10 4 cells per well in 96‐well plates. Cell proliferation was evaluated after 24–72 h. At the end of the experimental period, the sulforhodamine B (SRB) assay or a water‐soluble tetrazolium salt (WST‐1) assay was performed. Optical densities were determined in a microplate reader (Genius; TECAN). Data were analysed by Student’s t ‐test (comparison between cell types) and one‐way anova followed by Tukey test (time‐point intervals). Pearson’ correlation tests were performed to compare the two assays for each cell line. Results Both assays showed that DPSC had higher proliferation rates than HDPF. A positive significant correlation between the two colorimetric assays tested for both cell types DPSC (Pearson’s correlation coefficient = 0.847; P < 0.05) and HDPF (Pearson’s correlation coefficient = 0.775; P < 0.05). Conclusion Both tests demonstrated similar trends of cell proliferation, and thus are both appropriate for the evaluation of DPSC and HDPF. The choice of assay is therefore one of the practical applications. SRB stained plates can be dried and stored so may have utility in laboratories where data may require review or when access to analytical equipment is limited. WST‐1 assays have the benefit of both ease and speed and may have utility in laboratories requiring either high throughput or rapid analyses.