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Prostaglandin F 2α stimulates MEK‐ERK signalling but decreases the expression of alkaline phosphatase in dental pulp cells
Author(s) -
Chang M. C.,
Chen Y. J.,
Lee M. Y.,
Lin L. D.,
Wang T. M.,
Chan C. P.,
Tsai Y. L.,
Wang C. Y.,
Lin B. R.,
Jeng J. H.
Publication year - 2010
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2010.01699.x
Subject(s) - creb , pulp (tooth) , mapk/erk pathway , alkaline phosphatase , kinase , chemistry , receptor , phosphatase , microbiology and biotechnology , phosphorylation , biology , transcription factor , biochemistry , dentistry , medicine , enzyme , gene
Chang MC, Chen YJ, Lee MY, Lin LD, Wang TM, Chan CP, Tsai YL, Wang CY, Lin BR, Jeng JH. Prostaglandin F 2α stimulates MEK‐ERK signalling but decreases the expression of alkaline phosphatase in dental pulp cells. International Endodontic Journal , 43 , 461–468, 2010. Abstract Aim To study prostaglandin F 2α (PGF 2α ) receptor expression and downstream signalling in cultured human dental pulp cells and the effect of PGF 2α on the alkaline phosphatase (ALP) activity of dental pulp cells. Methodology Human dental pulp cells were cultured and exposed to PGF 2α . The expression of PGF 2α (FP) receptors was analysed by reverse transcriptase polymerase chain reaction (RT‐PCR) and Western blotting. The activation of extracellular regulated kinase (ERK) and cAMP responsive element binding protein/activating transcription factor‐1 (CREB/ATF‐1) signalling was determined by Western blotting. The expression of ALP in pulp cells after exposure to PGF 2α was evaluated by ALP staining and PCR. Results Dental pulp cells expressed FP receptor mRNA and protein. Exposure to PGF 2α revealed little cytotoxicity to pulp cells. PGF 2α induced both ERK and CREB/ATF‐1 phosphorylation in pulp cells. Exposure to PGF 2α (>1 μmol L −1 ) further decreased the ALP activity and mRNA expression. However, U0126 (an inhibitor of MEK1) showed little preventive effect on the decline of ALP activity in dental pulp cells by PGF 2α . Conclusion PGF 2α may potentially activate FP receptors leading to ERK/CREB‐ATF‐1 activation during its production in inflamed dental pulp. PGF 2α attenuated the ALP activity of pulp cells possibly via pathways not solely by MEK/ERK activation. PGF 2α is a contributing factor of pulpal inflammation by regulating the activities of pulp cells.