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Cytotoxicity of endodontic materials over 6‐weeks ex vivo
Author(s) -
Brackett M. G.,
Marshall A.,
Lockwood P. E.,
Lewis J. B.,
Messer R. L. W.,
Bouillaguet S.,
Wataha J. C.
Publication year - 2008
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2008.01471.x
Subject(s) - cytotoxicity , ex vivo , dentistry , in vivo , materials science , biomedical engineering , medicine , chemistry , in vitro , biology , biochemistry , microbiology and biotechnology
Aim To test the hypothesis that extending the time of a traditional ex vivo cytotoxicity test helps to identify trends in the behaviour of root core materials and sealers, which could ultimately aid in predicting their clinical safety and performance. Methodology Endodontic sealers and core specimens were initially tested in direct contact with L929 fibroblasts for 72 h. Cell response was estimated by measuring cellular succinate dehydrogenase activity relative to Teflon controls. Cytotoxicity (% of more active cells) was reassessed after 1, 3, 4 and 6 weeks, with the specimens stored in a physiologically balanced salt‐solution between tests. Results Distinct trends in cytotoxicity among both core materials and sealers were observed over the 6‐week test. Four of the six sealers and two of the three core materials showed cell viabilities of <30% of Teflon after 6 weeks (>70% cytotoxicity). Conclusions The current results suggest that some endodontic materials have an elevated biological risk for extended intervals.