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Ex vivo antimicrobial activity of several bleaching agents used during the walking bleach technique
Author(s) -
Oliveira D. P.,
Gomes B. P. F. A.,
Zaia A. A.,
SouzaFilho F. J.,
Ferraz C. C. R.
Publication year - 2008
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2008.01448.x
Subject(s) - antimicrobial , sodium perborate , enterococcus faecalis , bleach , chlorhexidine , chemistry , hydrogen peroxide , ex vivo , candida albicans , agar , cleanser , microbiology and biotechnology , brain heart infusion , biofilm , streptococcus mutans , food science , agar plate , dentistry , bacteria , in vitro , medicine , biology , biochemistry , escherichia coli , genetics , organic chemistry , gene
Aim To investigate ex vivo the antimicrobial activity of a paste of sodium perborate associated with various vehicles comparing it with 37% carbamide peroxide and 35% hydrogen peroxide. Methodology The antimicrobial activity of these agents was evaluated against three microorganisms: Enterococcus faecalis , Streptococcus mutans and Candida albicans . One millilitre of each tested substance was placed on the bottom of wells of 24‐well cell culture plates. Six wells were used for each time period and group. Two millilitres of the microbial suspension was ultrasonically mixed for 10 s with the bleaching pastes and placed in contact with them for 10, 30, 45 s; 1, 3, 5, 10, 20, 30 min; and 1 and 2 h. After each period of time, 1 mL from each well was transferred to tubes containing 2 mL of freshly prepared brain heart infusion agar + neutralizers. Agar plates were inoculated in appropriate gaseous conditions. Data were analysed statistically by the Kruskal–Wallis test with the level of significance set at P < 0.05. Results In all groups containing chlorhexidine (groups 3, 5 and 7), the antimicrobial activity of the bleaching paste was significantly increased when compared with groups with other kinds of vehicle (groups 1, 2, 4, 6 and 8). For all tested groups, the most resistant microorganism was E. faecalis. Conclusions Chlorhexidine when used as a vehicle for sodium perborate enhanced its antimicrobial activity.