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Biocompatibility of various root canal filling materials ex vivo
Author(s) -
Scotti R.,
Tiozzo R.,
Parisi C.,
Croce M. A.,
Baldissara P.
Publication year - 2008
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2008.01403.x
Subject(s) - viability assay , biocompatibility , incubation , ex vivo , filler (materials) , in vivo , materials science , andrology , chemistry , biomedical engineering , dentistry , in vitro , composite material , biology , medicine , biochemistry , microbiology and biotechnology , metallurgy
Aim To evaluate the biocompatibility of a resin‐based endodontic filler (RealSeal) using the indirect cytotoxicity test. Methodology Human gingival fibroblasts were cultured ex vivo . Pellets of the materials to be tested were incubated for 24, 48, and 72 h at 37 °C under sterile conditions to obtain their eluates. The fibroblasts were exposed to either diluted (50%) or undiluted eluates for 24 h. A culture medium with foetal calf serum was added to the control wells. Cell viability was estimated by 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide method. The data concerning cell viability were statistically analyzed using one‐way anova test and Bonferroni multiple comparisons test. Results Eluates obtained after 24 h of incubation with the resin filler did not reduce cellular viability. An increase in cellular viability, as compared with control cells, was observed in the gutta‐percha group. The undiluted eluate from the polyether material was cytotoxic, causing an 82 ± 4% decrease in cellular viability. Eluates obtained after 48 h of incubation with the resin filler increased cellular viability, whereas the polyether significantly reduced viability. Gutta‐percha did not cause any detectable change. After 72 h of incubation the eluate of the resin filler caused an increase in cellular viability, as did gutta‐percha, whereas polyether caused a significant decrease. Conclusions RealSeal resin filler was nontoxic in this laboratory model. Further investigations are necessary to verify its usefulness in clinical applications.