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Cytotoxicity analysis of EDTA and citric acid applied on murine resident macrophages culture
Author(s) -
Amaral K. F.,
Rogero M. M.,
Fock R. A.,
Borelli P.,
Gavini G.
Publication year - 2007
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2007.01220.x
Subject(s) - citric acid , cytotoxicity , in vivo , chemistry , chelation , toxicity , biochemistry , cytotoxic t cell , mtt assay , microbiology and biotechnology , in vitro , biology , inorganic chemistry , organic chemistry
Aim To assess the ex vivo cytotoxicity of EDTA and citric acid solutions on macrophages. Methodology The cytotoxicity of 17% EDTA and 15% citric acid was evaluated on murine macrophage cultures using MTT‐Tetrazolium method [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5 diphenyl tetrazolium bromide]. A total of 5 × 10 5 cells were plated in medium culture with 17% EDTA or 15% citric acid. Fresh medium was used as a control. Toxicity values were analysed statistically by anova and Tukey's test ( P < 0.05) at short (0, 6, 12, 24 h) and medium periods (1, 3, 5, 7 days), using ELISA absorbance. Results On the short term, both EDTA (0.253 nm) and citric acid (0.260 nm) exhibited cytotoxic effects on macrophage cultures ( P < 0.05). On the medium term, statistical differences were observed ( P < 0.05) between the groups. EDTA (0.158 nm) and citric acid (0.219 nm) were cytotoxic when compared with the control group; EDTA‐reduced macrophage viability significantly more than citric acid ( P < 0.05). Conclusions Both EDTA and citric acid had effects on macrophages cells ex vivo , but citric acid was less toxic in periods from 1 to 7 days of use.