Premium
Hemin nutritional stress inhibits bacterial invasion of radicular dentine by two endodontic anaerobes
Author(s) -
Love R. M.
Publication year - 2007
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2006.01186.x
Subject(s) - hemin , prevotella intermedia , dentinal tubule , microbiology and biotechnology , prevotella , bacteria , fusobacterium nucleatum , peptostreptococcus , chemistry , anaerobic bacteria , biology , dentistry , heme , dentin , porphyromonas gingivalis , medicine , biochemistry , enzyme , genetics
Aim To determine if anaerobic bacteria routinely found in infected dentine and root canals require the presence of heme in the environment in order for them to invade dentinal tubules. Methodology Noncarious, unrestored human teeth with single root canals were prepared for invasion experiments and soaked in either TSB‐M supplemented with hemin (5 μ g mL −1 ) ( n = 12 roots), TSB‐M media ( n = 12 roots) or TSB‐M media followed by hemin soak ( n = 12 roots) for 2 days, then inoculated with either Prevotella intermedia ATCC 25611 or Peptostreptococcus micros ATCC 33270 and incubated anaerobically for 14 days. Roots were prepared for light microscopy, stained with Brown and Brenn or antisera raised to the bacteria, and invasion within tubules assessed using a tubule invasion index (TI). Data were analysed using Student's t ‐test and Mann–Whitney U ‐test. Results Prevotella intermedia (TI = 0.7 ± 0.04) and P. micros (TI = 0.96 ± 0.08) showed low invasion when grown in the presence of hemin with cells generally restricted to the superficial 20 μ m of the tubules, whilst neither bacteria invaded tubules (TI = 0) when hemin was absent from the growth media ( P < 0.01). Conclusions Hemin was required in the growth medium for P. intermedia and P. micros to invade dentinal tubules.