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Cytotoxicity of Epiphany ® and Resilon ® with a root model
Author(s) -
Susini G.,
About I.,
TranHung L.,
Camps J.
Publication year - 2006
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2006.01167.x
Subject(s) - epiphany , root canal , cementum , cytotoxicity , dentistry , chemistry , nuclear chemistry , medicine , dentin , art , in vitro , biochemistry , visual arts
Aim To record the cytotoxicity of Resilon and Epiphany (Pentron clinical technologies, Wallingford, CT, USA) using a root model. Methodology Thirty teeth with single roots were sectioned at the enamel–cementum junction, the root canals prepared and each root then sterilized before filling with the lateral condensation technique using one of three filling materials ( n = 10 per group): Resilon and Epiphany, Sealite (Septodont, Pierre Rolland, Merignac, France) and gutta‐percha, Roekoseal Automix (Coltène/Whaledent, Langenau, Germany) and gutta‐percha. The roots were stored at 37 °C in an incubator to allow for setting of the root filling materials. The apices of the roots were dipped in 1 mL of MEM culture medium for 1, 2, 7 and 30 days renewing the medium every day. After 24 h contact between the medium and the filled roots, the medium was used to measure the cytotoxicity on mouse fibroblasts L 929 with the MTT assay that recorded the mitochondrial activity of the target cells. An additional test according to ISO 10993‐5 standards was undertaken to compare Resilon and Epiphany. Results The root model showed no statistically significant differences between the sealers at 7 and 30 days (NS). Epiphany and Resilon were the most cytotoxic materials at 1 and 2 days ( P < 0.001). Unlike Epiphany, Resilon was not cytotoxic when tested according to ISO 10993‐5 standards. Conclusions The cytotoxicity of Resilon + Epiphany, due mainly to Epiphany, decreased after 2 days to reach a level comparable with commonly used root canal sealers.