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Biodegradable porous calcium polyphosphate scaffolds for the three‐dimensional culture of dental pulp cells
Author(s) -
Wang F.M.,
Qiu K.,
Hu T.,
Wan C.X.,
Zhou X.D.,
Gutmann J. L.
Publication year - 2006
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2006.01114.x
Subject(s) - polyphosphate , calcium , materials science , pulp (tooth) , dental pulp stem cells , dentistry , porosity , composite material , medicine , chemistry , in vitro , biochemistry , metallurgy , phosphate
Aim To develop a three‐dimensional culture model of human dental pulp cells (DPCs) with biodegradable porous calcium polyphosphate (CPP) scaffolds. Methodology Human DPCs were isolated from three donors. The 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) assay was used to evaluate the cytotoxicity of CPP compared with hydroxyapatite (HA) and β ‐tricalcium phosphate ( β ‐TCP). Values were analysed using unpaired t ‐tests. Cells were seeded onto porous CPP scaffolds with pore sizes in the range of 200–300 μ m. The nature of cellular adaptation in the three‐dimensional culture model was then evaluated visually by scanning electronic microscopy (SEM) and confocal laser scanning microscopy (CLSM). The apoptotic property of cells on the scaffolds was also assessed by DNA staining with CLSM. Results The cytotoxicity assay indicated that there was no significant difference between CPP and HA for each donor's original cells ( P > 0.05). Calcium polyphosphate had no cytotoxic effect on DPCs, whilst SEMs showed that cells successfully adhered to CPP scaffolds and spread amongst pores. On the cell surface, fine processes and matrix secretory granules were found. Confocal laser scanning microscopy showed that cells took on a three‐dimensional structure with signs of vitality. Conclusion Porous CPP scaffolds are promising for the establishment of a three‐dimensional culture model of DPCs.