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Ex vivo biocompatibility tests of regular and white forms of mineral trioxide aggregate
Author(s) -
Ribeiro D. A.,
Matsumoto M. A.,
Duarte M. A. H.,
Marques M. E. A.,
Salvadori D. M. F.
Publication year - 2006
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2005.01043.x
Subject(s) - mineral trioxide aggregate , trypan blue , comet assay , genotoxicity , viability assay , cytotoxicity , in vivo , chemistry , microbiology and biotechnology , ex vivo , biocompatibility , chromatography , biology , medicine , dna damage , toxicity , biochemistry , apoptosis , in vitro , dentistry , dna , organic chemistry
Aim To examine the genotoxicity and cytotoxicity of regular and white mineral trioxide aggregate (MTA) ex vivo by the single‐cell gel (comet) assay and trypan blue exclusion test, respectively. Methodology Aliquots of 1 × 10 4 Chinese hamster ovary cells were incubated at 37 °C for 3 h with grey and white forms of MTA at final concentrations ranging from 1 to 1000 μ g mL −1 . The negative control group was treated with vehicle control phosphate buffer solution for 3 h at 37 °C and the positive control group was treated with methyl metasulfonate (at 1 μ g mL −1 ) for 1 h at 37 °C. After incubation, the cells were centrifuged at 180 g for 5 min and washed twice with fresh medium and resuspended with fresh medium. Each individual treatment was repeated three times consecutively to ensure reproducibility. Parameters from single‐cell gel (comet) and cytotoxicity assays were assessed by the Kruskal–Wallis nonparametric test. Results Neither compounds produced genotoxic effects with respect to the single‐cell gel (comet) assay in all concentrations evaluated. In the same way, the dose–response relationships of all compounds tested at concentrations ranging from 1 to 1000 μ g mL −1 on cell viability assessed by the trypan blue assay displayed no statistically significant differences ( P > 0.05) for either endodontic material. Conclusions Regular (grey) and white MTA are not genotoxins and do not induce cellular death.