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Heat shock induces the synthesis of the inflammatory mediator leukotriene B 4 in human pulp cells
Author(s) -
Eberhard J.,
Zahl A.,
Dommisch H.,
Winter J.,
Acil Y.,
Jepsen S.
Publication year - 2005
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2005.01032.x
Subject(s) - dental pulp stem cells , mediator , leukotriene b4 , pulp (tooth) , stimulation , arachidonic acid , chemistry , microbiology and biotechnology , inflammation , biochemistry , biology , medicine , immunology , dentistry , endocrinology , in vitro , enzyme
Aim To measure the synthesis of leukotriene B 4 (LTB 4 ) in cultures of human dental pulp cells induced by heat shock. Methodology Primary pulp cells (PC) and dental pulp stem cells (DPSC) were cultivated under appropriate conditions. For the characterization of PC the expression of dentine sialophosphoprotein (DSPP) was evaluated by reverse transcription‐polymerase chain reaction. Thermal stimulation of cell cultures was performed at temperatures of 37, 38, 39, 40, 42 and 45 °C for stimulation times of 5 and 30 s. LTB 4 was quantified by reversed‐phase high‐performance chromatography and differences between the LTB 4 concentrations of controls and heat stimulated cells were analysed with Friedman analysis of variances by ranks and multiple comparisons ( P < 0.05). Results Both cell cultures expressed DSPP under the conditions of the present experiment. The analysis revealed significantly enhanced LTB 4 synthesis following thermal stimulations at 38, 39, 40, 42 and 45 °C compared with unstimulated controls for both PC and DPSC. Conclusion The present study demonstrated the capability of pulp cells to synthesize the arachidonic acid mediator LTB 4 in response to heat shock. LTB 4 has the capacity to induce inflammatory reactions and to sensitise afferent nociceptive nerve endings. LTB 4 synthesis is induced by minor temperature changes, which are relevant for various clinical situations.