Premium
Effects of 2% chlorhexidine and 5.25% sodium hypochlorite on gutta‐percha cones studied by atomic force microscopy
Author(s) -
Valois C. R. A.,
Silva L. P.,
Azevedo R. B.
Publication year - 2005
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2005.00940.x
Subject(s) - sodium hypochlorite , chlorhexidine , atomic force microscopy , gutta percha , materials science , dentistry , elasticity (physics) , chemistry , microscopy , nuclear chemistry , composite material , medicine , root canal , nanotechnology , pathology , organic chemistry
Aim To compare the effects of 2% chlorhexidine (CHX) and 5.25% sodium hypochlorite (NaOCl) on gutta‐percha (GP) cone structure using atomic force microscopy (AFM). Methodology Two standardized GP cones were sectioned 3 mm from the tip, attached to a glass base and immersed in 2% CHX or 5.25% NaOCl for 1, 5, 10, 20 and 30 min. Untreated GP cones were used as control. Topography and elasticity analyses were performed on 12 different regions located between 1 and 2 mm from the tip. Root mean square (RMS) parameters for contact mode imaging and force modulation microscopy variations were measured. The differences between RMS values were tested by anova with Fisher's protected LSD test for multiple comparisons. Results There was no deterioration in the topography and physical properties studied when 2% CHX was used in comparison with the control ( P < 0.05). The RMS parameter for topography increased after 10 min of 5.25% NaOCl exposure in comparison with the control ( P < 0.05). In addition, 5.25% NaOCl increased the elasticity of the GP cone after an immersion time of 1 min in comparison with the control ( P < 0.05). Conclusions Two per cent CHX did not change GP cone structure following up to 30 min exposure. Conversely, 5.25% NaOCl caused elastic changes after 1 min exposure.