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Influence of the pulpal components on human dentine permeability in vitro
Author(s) -
Puapichartdumrong P.,
Ikeda H.,
Suda H.
Publication year - 2005
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2004.00886.x
Subject(s) - smear layer , pulp (tooth) , molar , enamel paint , materials science , scanning electron microscope , dentistry , saline , chemistry , composite material , medicine , endocrinology
Aim To examine the influence of the retained pulpal components on permeability of human dentine by monitoring drug diffusion. Methodology Twelve intact dentine discs were prepared from freshly extracted human third molars. The dentine surface on the enamel side was etched with 10% polyacrylic acid for 30 s. The drug diffusion test was carried out before and after removal of the retained pulpal components. Each dentine disc was inserted between two plastic chambers; enamel‐ and pulpal‐side chambers, which were filled with 0.05 mol L −1 naproxen sodium (NA) and phosphate‐buffered saline (PBS), respectively. After 10 min, the solution on the pulpal‐side chamber was collected to determine the concentration of NA using a spectrophotometer. To remove the retained pulpal components and residual NA, the pulp chamber of each disc was washed out with PBS and placed in an ultrasonic cleaner. After removal of these components and the residual NA, the drug diffusion test was repeated. The inner surface of the pulp chamber was observed using scanning electron microscopy (SEM) before and after the removal of the retained pulpal components. Results The amount of NA that diffused through dentine into the pulp was significantly higher after the pulp chamber was washed out with PBS (paired t ‐test, P < 0.05). SEM observation demonstrated the presence of the retained pulpal components, odontoblastic layer and some parts of subodontoblastic zone, covering the surface of predentine. These components were removed after the pulp chamber was washed out with PBS followed by cleaning in an ultrasonic cleaner. Conclusions The presence of retained pulpal components had a significant influence on drug diffusion through dentine discs.