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Induction of vascular endothelial growth factor expression in human pulp fibroblasts stimulated with black‐pigmented Bacteroides
Author(s) -
Yang L.C.,
Tsai C.H.,
Huang F.M.,
Su Y.F.,
Lai C.C.,
Liu C.M.,
Chang Y.C.
Publication year - 2004
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2004.00847.x
Subject(s) - porphyromonas gingivalis , pulp (tooth) , prevotella intermedia , vascular endothelial growth factor , bacteroides , gene expression , microbiology and biotechnology , biology , vegf receptors , fibroblast , bacteroidaceae , gene , bacteria , cell culture , cancer research , pathology , medicine , genetics
Aim To investigate the effect of black‐pigmented Bacteroides on the expression of vascular endothelial growth factor (VEGF) gene in human pulp fibroblasts. Methodology The supernatants of Porphyromonas endodontalis , Porphyromonas gingivalis and Prevotella intermedia were used to evaluate VEGF gene expression in human pulp fibroblasts. The levels of mRNAs were measured by the quantitative reverse‐transcriptase polymerase chain reaction analysis. Results Black‐pigmented Bacteroides induced significantly high levels of VEGF mRNA gene expression in human pulp fibroblasts ( P < 0.05). In addition, the expression of VEGF depended on the bacteria tested. Conclusions Black‐pigmented Bacteroides may be involved in developing pulpal disease through the stimulation of VEGF production that would lead to the expansion of the vascular network coincident to progression of the inflammation.