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Expression of transforming growth factor‐beta 1 (TGF‐beta 1) in odontogenic cysts
Author(s) -
Piattelli A.,
Rubini C.,
Fioroni M.,
Favero L.,
Strocchi R.
Publication year - 2004
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2004.00739.x
Subject(s) - stromal cell , basal (medicine) , epithelium , pathology , transforming growth factor beta , immunohistochemistry , transforming growth factor , beta (programming language) , biology , medicine , endocrinology , programming language , computer science , insulin
Aim To evaluate the positivity to transforming growth factor‐beta 1 (TGF‐beta 1) in different types of odontogenic cysts. Methodology A total of 30 radicular cysts (RCs), 27 follicular cysts (FCs) and 28 odontogenic keratocysts (OKCs) were evaluated for immunohistochemical analysis of TGF‐beta 1. TGF‐beta 1 was evaluated in blood vessels, stromal cells (fibroblasts) and pluristratified squamous epithelium. TGF‐beta 1 expression was determined by evaluating the number of positive elements. TGF‐beta 1 expression was determined by evaluating 1000 cells in the pluristratified squamous epithelium (500 in the basal and parabasal layers, and 500 in the superficial layer) and 500 cells (the fibroblasts in the stroma) for each specimen, and counting the number of positive cells. The number of positive vessels was evaluated in 10 high power fields (HPF). The Chi‐square test was used to evaluate differences between the two groups (RC + FC and OKC). A P ‐value <0.05 was considered to indicate statistical significance. Results A higher and statistically significant positivity was found in the basal–suprabasal epithelial layers ( P = 0.0011), superficial epithelium ( P = 0.053) and stromal cells ( P = 0.0002) of orthokeratotic and parakeratotic OKC as compared with RC and FC. Conclusions These differences suggest that control of the cell cycle may be abnormal in orthokeratotic OKCs. These OKCs may have an intrinsic growth potential not present in other cyst types.