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Lymphangiogenesis in human dental pulp
Author(s) -
Pimenta F. J. G. S.,
Sá A. R.,
Gomez R. S.
Publication year - 2003
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2003.00728.x
Subject(s) - lymphangiogenesis , cd31 , inflammation , pulp (tooth) , lymphatic system , cd34 , medicine , pathology , immunostaining , lymphatic endothelium , stain , dentistry , immunohistochemistry , biology , staining , immunology , microbiology and biotechnology , cancer , metastasis , stem cell
Aim To investigate the impact of inflammation on lymphangiogenesis in human dental pulp. Methodology Eleven samples of dental pulp without inflammation and 11 dental pulps with moderate to intense mononuclear cell inflammatory infiltrate associated with dentine caries were selected. The streptavidin–biotin complex stain was used to detect CD31, vascular endothelial growth factor receptor‐3 (VEGFR‐3) and α‐smooth muscle actin. The number of lymphatic vessels was obtained by counting the number of vessels positive for CD31 and VEGFR‐3 and negative for α‐smooth muscle actin. Results The results demonstrated that the mean number (±SD) of vessels positive for CD31 and VEGFR‐3 (lymphatic vessels) in the group with inflammation (6.09 ± 1.81) was statistically higher ( P = 0.0123) than the mean number in the group without inflammation (3.73 ± 2.20). Conclusion Increased co‐immunostaining of CD31 and VEGF‐3 in vessels associated with human dental pulp inflammation occurred, which suggests lymphangiogenesis.