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Detection of epidermal growth factor receptor in inflammatory periapical lesions
Author(s) -
LIN L. M.,
WANG S.L.,
WUWANG C.,
CHANG K.M.,
LEUNG C.
Publication year - 1996
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.1996.tb01366.x
Subject(s) - immunoperoxidase , pathology , immunohistochemistry , epidermal growth factor receptor , staining , radicular cyst , epidermal growth factor , cell , biology , cyst , medicine , receptor , immunology , monoclonal antibody , antibody , genetics
Summary Epithelial cell proliferation is often observed in periapical lesions of endodontic origin. The mechanisms which stimulate the epithelial cell rests of Malassez to proliferate are not understood fully. Fifteen inflammatory periapical lesions (10 granulomas and five cysts) obtained from periapical surgery and six additional periapical lesions (four granulomas and two cysts) collected from extracted teeth were examined using immunohistochemical staining and 125 I‐EGF (epidermal growth factor) binding assay to detect the presence of epidermal growth factor receptor. The results indicated that the periapical lesions without epithelial cell proliferation had a weak immunoperoxidase staining or low specific binding of i 25 I‐EGF. In contrast, the periapical lesions with epithelial cell proliferation and cyst formation exhibited a strong immunoperoxidase staining in the epithelial cells or high specific binding of 125 I‐EGF.